FastQCFastQC Report
Wed 25 May 2016
SRR1779629_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779629_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences558743
Sequences flagged as poor quality0
Sequence length76
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG243634.36032308234734No Hit
CTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTG11100.1986602069287669No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTCG305.877426E-446.665659
CGTATCA2400.040.83244356
TCATTAA2850.036.84130560
CATTAAA2850.035.61326261
ATCATTA2850.035.61326259
TTTATAC400.00241863134.9992373
CTATTGT400.00241863134.9992374
TAGATCT3100.033.8702339
TATCATT2850.033.15717358
ATTAAAA3200.031.7180662
GTATCAT3200.030.62433257
CCGTATC3100.030.48320855
GCCGTAT3100.030.48320854
TAAACCT500.00720801327.9993924
GTTTTGT500.00720801327.9993921
CAGTATT500.00720801327.9993924
AGATCTC3700.027.43183540
GTAGATC4000.027.1244138
TGTAGAT4050.026.79193937
ATCTTAC808.6641296E-526.24942859
Produced by FastQC (version 0.11.5)