FastQCFastQC Report
Wed 25 May 2016
SRR1779629_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779629_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences558743
Sequences flagged as poor quality0
Sequence length76
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT137202.455511746903317No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG26930.4819747182515038No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20730.3710113594264268No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC15440.2763345581063208No Hit
ATTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT14500.25951108112316396No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG16150.059.57367770
CGTCTTA407.405906E-752.57309348
GGTCCTA200.00695625952.48838437
CGTTACT200.006958721752.483692
TGGGTAG200.00696118552.47899224
TCGTATG31250.048.8386840
CCGTCTT29500.048.7117947
GTATGCC30300.048.56497642
TTGAAAA31250.048.16658858
ATTATAC3200.048.1143531
CTCGTAT31000.048.0904439
TGAAAAA32500.047.92963459
CGTATGC31350.047.82389541
CTTGAAA30900.047.81017757
GTCTTCT30150.047.7564649
GAAAAAA33850.047.6556360
TCTCGTA31300.047.62524838
TATGCCG31050.047.3919143
TCTGCTT29950.047.37777753
GCTTGAA31600.046.7678456
Produced by FastQC (version 0.11.5)