FastQCFastQC Report
Wed 25 May 2016
SRR1779628_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779628_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences413929
Sequences flagged as poor quality0
Sequence length76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG192924.660702680894549No Hit
CTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTG6380.15413271358131467No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGA501.7085971E-435.05
GCGTACG652.1007887E-532.3076931
TACACTA450.00430530731.1111135
GTATCAT1750.030.057
GACATTT703.4855046E-530.042
AGTGTCC604.9788493E-429.16666651
CTATACA755.5795583E-528.0000024
CGTATCA2000.027.99999856
ACTCTAA1004.413123E-727.99999856
TCCTATA500.007205124527.9999982
CTACCGA500.007205124527.99999869
TAGATCT2200.027.04545439
TAGCGTC657.950686E-426.9230777
ATTACAC657.950686E-426.9230773
GTTAGCA657.950686E-426.92307722
GGACATT657.950686E-426.92307741
GCCGTAT1850.026.48648654
GAATAGA1101.014274E-625.4545441
ATCATTA1951.8189894E-1225.12820459
CACTTAA700.001225228525.055
Produced by FastQC (version 0.11.5)