FastQCFastQC Report
Wed 25 May 2016
SRR1779628_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779628_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences413929
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT109432.643690101442518No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG19190.4636060773707569No Hit
ATTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT13050.3152714595981437No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12170.29401177496623815No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC11820.2855562185785485No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGAG150.002222693570.0095457
AGGGGGG13000.055.72490370
TCGTATG23750.050.5481540
GTATGCC23250.050.44917342
CGTATGC23850.050.34229741
CTCGTAT23200.050.2378539
CTTGAAA23350.049.1715957
TTGAAAA23750.049.07444458
ATTATAC3800.048.8106541
TGAAAAA25350.048.73842659
TCTCGTA23900.048.6141338
CCGTCTT22100.048.32727447
TATGCCG24000.048.2949243
GTCTTCT22100.047.8345849
TAAGATC23750.047.7423433
GCTTGAA23950.047.6474256
TCTGCTT22250.047.51783853
ATCTCGT23750.047.44763637
GAAAAAA26850.047.31345760
GCCGTCT23100.047.29631846
Produced by FastQC (version 0.11.5)