FastQCFastQC Report
Wed 25 May 2016
SRR1779627_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779627_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences446988
Sequences flagged as poor quality0
Sequence length76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG170453.8133014756548276No Hit
CTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTG5340.11946629439716502No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAATAC305.8755255E-446.6666683
GATATTA400.00241785735.017
CGTATCA1500.032.66666856
TATTACG652.1011627E-532.3076934
TGGTATG450.00430567231.11111348
TCATTAA1900.029.47368660
TACGGAT604.979477E-429.1666687
GTATCAT1800.029.16666857
GATCCTA500.007205728428.0000021
TAGATCT2000.028.00000239
ATCATTA2100.026.66666659
AACCGTA700.001225382125.07
CATTAAA2400.024.79166861
GCTTAGA750.001831286823.3333341
GTATAAG750.001831286823.3333341
TAAGACG750.001831286823.33333453
GCCGTAT1854.0199666E-1022.70270354
GTGCCTA1003.928444E-421.00000211
TACAGGC1205.707681E-520.4166682
ACATATA1601.1830925E-619.687544
Produced by FastQC (version 0.11.5)