FastQCFastQC Report
Wed 25 May 2016
SRR1779627_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779627_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences446988
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT81471.8226440083402689No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG12310.2753988921402812No Hit
ATTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT9920.2219298952097148No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9690.2167843432038444No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC8240.18434499360161793No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG8950.052.7785370
CTCGTAT16700.052.59007339
TCGTATG16700.052.59007340
CCGTCTT14200.052.0798947
CGTATGC16300.051.99519341
TCTCGTA17400.051.68094638
CTTGAAA15400.051.3780157
TTGAAAA15850.050.80285658
GTATGCC17750.050.7356742
TCTGCTT11750.050.38270653
TATGCCG17900.050.31051343
TCTTCTG11550.050.01399250
GCCGTCT14750.049.90030346
TGAAAAA21350.049.85001459
GTCTTCT11750.048.8647349
ATCTCGT18750.048.70637537
GCTTGAA16000.048.13846656
CGTCTTA2550.048.10650348
GGATCTC19900.047.6499935
CTGCTTG12350.047.6406654