FastQCFastQC Report
Wed 25 May 2016
SRR1779626_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779626_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences529426
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG231884.379837786583961No Hit
CTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTG22520.4253663401495204No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT3850.044.54449554
CGTATCA3950.043.41678256
GTATCAT4250.040.3520757
CCGTATC4550.037.69149855
TCTCGGT4800.037.186743
TCATTAA4700.035.74390860
AGATCTC4950.035.3527740
GGTCTAA400.002416273835.005861
TAGATCT5100.034.99924539
TCGGTGG5450.034.0359645
GTAGATC5050.033.95966338
ATCATTA4950.033.9386659
TATCATT5150.032.62065558
CGCCGTA5400.031.75857453
CATTAAA5350.031.40119261
CGGTGGT5850.031.1104446
TGTAGAT5900.030.25358637
CTGTGTA6000.029.7493634
GTGTAGA6450.029.30169536
TGGTCGC6200.028.22519949