FastQCFastQC Report
Wed 25 May 2016
SRR1779626_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779626_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences529426
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT130542.4656892559111188No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38400.7253138304503368No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG24790.46824296502249607No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC14950.28238129596959727No Hit
ATTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT14750.27860361976933506No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG14500.053.5679970
ACGTATG200.006951533752.4970240
CGTCTTA1350.051.9175848
CGTATGC24150.051.50474541
TCGTATG25650.050.89380340
CTCGTAT25750.050.82726739
GTATGCC24150.050.34884342
TATGCCG24250.050.00144643
TCTCGTA26750.049.70741738
TGAAAAA27350.049.5312359
CCGTCTT21800.049.51690747
GTCTTCT21250.049.28151349
TTGAAAA25100.048.6717858
CTTGAAA24250.048.5104357
TCTTCTG21500.048.3826650
ATCTCGT27700.048.2552937
GCTTGAA24450.047.2589156
CGTCTTC22500.047.1930848
GCCGTCT23150.046.93210246
ATGCCGT25600.046.81708544
Produced by FastQC (version 0.11.5)