FastQCFastQC Report
Wed 25 May 2016
SRR1779625_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779625_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences548173
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG196613.5866414434859073No Hit
CTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTG8080.14739872266601967No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGGA200.00695010852.527
CGATTGC305.8766984E-446.66666441
GCCGTAT1300.043.07692354
CCTTACG350.001255194240.01
TTAGAGT806.2409526E-835.04
TTAGAAC400.002418333435.03
GTAAACG400.002418333435.049
CGACGTT450.004306514731.11111314
TACGCAC703.4875477E-530.040
TTACTCG604.980933E-429.16666619
TGCGTGG604.980933E-429.16666612
CGTATCA1950.028.71794956
AAGTTGC755.582814E-528.00000227
TAAACGG500.00720713227.99999850
GTTACCA500.00720713227.99999822
CGGTGGT2400.027.70833246
CGCCGTA2050.027.31707453
TGGTCGC2350.026.80851249
TAGACGT808.6625565E-526.254
CCGTATC2150.026.04651355
Produced by FastQC (version 0.11.5)