FastQCFastQC Report
Wed 25 May 2016
SRR1779625_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779625_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences548173
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT110402.0139627453376945No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG20830.37998952885311754No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14960.27290654592619484No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC12560.2291247471145058No Hit
ATTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT12370.22565868804191377No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG14100.053.3525970
AGCGAAT200.00695845852.4840633
CCGTCTT20850.050.0864647
CGTATGC22600.050.0480241
TCGTATG22900.049.66193840
GCCGTCT21450.049.5023146
TATGCCG22450.049.32205643
CGTCTTC21400.049.29045548
GTATGCC22850.048.76535442
CTTGAAA22550.048.7391357
CTCGTAT23200.048.71809839
GTCTTCT21350.048.22607849
TTGAAAA23200.047.97707758
ATGCCGT23150.047.8306844
TCTTCTG21650.047.71957850
TGCCGTC23050.047.58647545
TCTCGTA24600.047.2257438
GCCGTAT305.8406475E-446.72495346
ATCGTAT305.882855E-446.6567539
CACGTAT305.882855E-446.6567539
Produced by FastQC (version 0.11.5)