FastQCFastQC Report
Wed 25 May 2016
SRR1779624_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779624_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences404557
Sequences flagged as poor quality0
Sequence length76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG208445.152302394965357No Hit
CTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTG10290.25435229151887123No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAATTA150.002223859570.05
GCCTATA200.006948797552.547
TCATTAA1550.049.67741860
CATTAAA1550.049.67741861
TATCATT1650.048.7878858
ATTAAAA1650.048.7878862
ATCATTA1700.047.3529459
AAGTATG404.5895024E-543.751
GTATCAT1950.041.28204757
CGTATCA2050.039.2682956
TTAAAAA2200.036.5909163
TAGATCT2150.035.81395339
ACTATTA652.1006717E-532.30769346
GCCGTAT2500.032.254
AGACTAT552.990051E-431.81818444
AAACAGG552.990051E-431.8181841
GTTGGAT552.990051E-431.81818416
GCGTATG552.990051E-431.8181841
CCGTATC2550.031.56862855
TCGTACA450.004305192731.11111325
Produced by FastQC (version 0.11.5)