FastQCFastQC Report
Wed 25 May 2016
SRR1779624_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779624_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences404557
Sequences flagged as poor quality0
Sequence length76
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT119152.945196844943976No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG22830.5643209733115482No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18040.44591985801753525No Hit
ATTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT15010.3710231191154769No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC12600.31145178553331176No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTAAG150.002225479669.9871534
AATAAGT150.002225479669.9871534
TAGTATG401.0664735E-861.23875440
CTATACA352.972356E-759.9963951
GTCTTAA308.342662E-658.36590249
ATTATAC3300.057.2692871
AGGGGGG13250.057.04612770
TCTTAAG252.3825976E-456.03126550
ATGTAAT200.006930080752.53581243
GTCGTAA200.006950446452.496851
GGTAATA200.00695384552.490362
GGGCTTA200.00695384552.4903624
TATTGTT200.00695384552.4903618
CTAGTAT407.481667E-752.4903639
CCATACT200.00695384552.490364
ATAAGTC200.00695384552.4903635
GAAAAAA26700.051.50739360
TCTGCTT22650.051.18279353
TGAAAAA25850.051.1768159
TCGTATG25100.051.02648540
Produced by FastQC (version 0.11.5)