FastQCFastQC Report
Wed 25 May 2016
SRR1779623_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779623_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1003610
Sequences flagged as poor quality0
Sequence length76
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG217882.1709628242046213No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTAC404.5945235E-543.749781
GTGTAAC350.001255723239.99981
CGTATCA2100.036.66648556
TATCATT2350.032.76742658
CATTAAA2500.032.20144761
CGTTAAT450.00430830931.1109582
TCATTAA2900.028.96681660
ATTACGT500.00721011527.9998629
ACGTAAG657.9588586E-426.9229437
ATCATTA3050.026.39462759
ATTAAAA3350.026.12057362
GTATCAT3000.025.66781657
TAAACGT851.3089029E-424.705764
CCGTATC3400.024.7057655
GTACTGA1001.3985866E-524.4998781
CTACTCT29450.023.88782770
TATAGAG1652.6611815E-923.3332182
TAAGGTT750.00183292323.3332166
CTCTAGG750.00183292323.3332162
GCCGTAT3550.022.67594354
Produced by FastQC (version 0.11.5)