FastQCFastQC Report
Wed 25 May 2016
SRR1779623_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779623_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1003610
Sequences flagged as poor quality0
Sequence length76
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT116541.1612080389792847TruSeq Adapter, Index 5 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG20240.20167196420920477TruSeq Adapter, Index 5 (95% over 22bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16050.15992267912834668No Hit
ATTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT12530.12484929404848497No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC11840.11797411345044391RNA PCR Primer, Index 5 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG13800.054.008970
TCGTATG25100.049.6345840
CGTATGC25000.049.582841
CTCGTAT24750.048.49593439
GTATGCC25300.048.4678342
TGAAAAA28400.047.56859259
TTGAAAA25200.047.49817358
GAAAAAA29400.047.36968660
CCGTCTT23700.046.87093747
TCTCGTA26300.046.4361438
CTTGAAA25200.046.10933757
GTCTTCT23800.045.6114949
CGTCTTA1100.044.60152448
ATTATAC4650.044.4002571
TATCGTA1500.044.32500538
GCCGTCT25700.044.03930746
TATGCCG27850.044.0300243
GCTTGAA26550.043.6373256
TCTTCTG25250.042.43747350
TGCTTGA25850.042.1234955
Produced by FastQC (version 0.11.5)