FastQCFastQC Report
Wed 25 May 2016
SRR1779622_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779622_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences274754
Sequences flagged as poor quality0
Sequence length76
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG203427.403713867677995No Hit
CTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTG14510.5281087809458642No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTTAT252.3878252E-455.99999660
TTTGACA200.00694643652.530
ACTACTT305.871552E-446.6666648
CGTATCA3050.041.31147856
GTATCAT3100.040.6451657
GCCGTAT3100.040.6451654
CATTAAA3350.039.70149261
GATTCGA459.1836264E-538.8888936
CCGTATC3450.037.53623255
TCATTAA3350.036.5671660
ATCATTA3400.036.0294159
TATCATT3350.035.5223958
CATGGAA400.002416241435.047
CATTGAG601.2131861E-535.02
GGCCCTA400.002416241435.020
TTTGGTC400.002416241435.028
TAGATCT4100.035.039
CGCCGTA3800.035.053
GGTAGAC400.002416241435.045
GTAGATC4250.032.94117738
Produced by FastQC (version 0.11.5)