FastQCFastQC Report
Wed 25 May 2016
SRR1779622_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779622_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences274754
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT70172.5539209620242107No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT52351.9053407775682973No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24650.8971661923029329No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG20760.7555849960328148No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC5810.2114618895448292No Hit
ATCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC5080.18489266762267337No Hit
ATTAAACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT3820.13903346266114416No Hit
CTTAAACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT2820.10263726824723206No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTATA301.0221447E-769.992542
ATTATAC10750.061.8538741
AGGGGGG13850.060.64335670
TATCGTA1100.057.2666238
ATTAAAC1850.054.859021
ATATCGT1150.054.77676837
CCGTCTT21250.052.90346547
CCATTAC200.00693936652.51351542
TCTAGAA200.006944364452.50395657
CGTAAAC200.00694936552.49440432
TCTTTAT407.468825E-752.4944041
TCAGTAA200.00694936552.49440410
GTCTTCT22150.052.3159549
TCGTATG23350.052.15717740
GTATGCC22300.052.1210442
CTTGAAA22500.052.11504457
TCTTCTG22250.052.08082250
CGTCTTC22350.052.02337648
CGTATGC22300.051.9545941
TTGAAAA23250.051.78885358