FastQCFastQC Report
Wed 25 May 2016
SRR1779621_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779621_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences339280
Sequences flagged as poor quality0
Sequence length76
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG173755.12113888233907No Hit
CTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTG10700.31537373261023344No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTTA150.002223549270.08
CGTTAAC200.00694783552.510
TATATTC305.873513E-446.66666446
GCCGTAT2700.041.4814854
ATCATTA2750.040.72727259
TAGATCT2650.039.62264339
ATTAAGC459.188193E-538.8888853
CGTATCA2800.038.7556
CATTAAA2800.038.7561
CCGTATC2850.038.07017555
TATCATT2800.037.558
GTATCAT3000.037.33333257
CGCCGTA3000.036.16666853
TCATTAA3100.035.00000460
AATGATT400.002417038435.023
TGTAGAT3350.033.4328437
TCTCGGT3000.032.66666843
AGATCTC3250.032.3076940
GTAGATC3300.031.8181838
GTAGATA450.00430422431.11110938