FastQCFastQC Report
Wed 25 May 2016
SRR1779621_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779621_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences339280
Sequences flagged as poor quality0
Sequence length76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT65791.9391063428436688No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT39201.15538788021693No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18720.5517566611648196No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG15790.4653973119547276No Hit
ATCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC4510.1329285545861825No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC4360.12850742749351568No Hit
ATTAAACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT3520.10374911577458147No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTATAC10050.063.0215571
AGGGGGG11800.058.419970
CGTATAT252.391114E-455.98820534
CGTATGA1150.054.80338741
CCAAAGG200.006953622752.488959
TCGTATG20750.050.9290340
CTCGTAT20750.050.76039539
CGTATGC19900.050.67247441
CTTGAAA19900.050.30574457
GTATGCC19350.050.15194342
TTGAAAA20350.050.05335658
TATGCCG19400.050.02268643
ACGTATG352.0869344E-549.9894740
TCTCGTA21600.049.73489438
TGAAAAA21700.049.68160659
GTATCTC21000.049.65620835
CCGTCTT18100.049.5653947
TATCTCG21300.049.44968436
CGTATCT20950.049.44066234
ATCTCGT21800.048.9575837
Produced by FastQC (version 0.11.5)