FastQCFastQC Report
Wed 25 May 2016
SRR1779620_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779620_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences476400
Sequences flagged as poor quality0
Sequence length76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG132582.7829554995801846No Hit
CTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTG6190.1299328295549958No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACTTG400.002418265734.99926456
GTATGCG450.004306396531.1104562
ACCTTAG450.004306396531.1104561
GTTATAC450.004306396531.1104561
TCCTATA604.9805763E-429.1660562
TCTATAC700.001225650524.9994743
CTACAGA851.3076351E-424.7053641
CGTATCA1553.4986442E-822.58017256
ACTAAAC1253.086292E-622.399533
AGATACC1102.9192981E-522.2722627
TATGCGC952.778491E-422.10483
TTACATC952.778491E-422.10484
ATCATTA1604.8967195E-821.8745459
TACACCG1304.332931E-621.540275
GTCCTAG1304.3368764E-621.538011
GCTTACA1003.929526E-420.999562
GGTTTGG1205.705004E-520.4183831
GTACAAG1205.7096164E-520.4162391
TATCATT1751.2618875E-719.9995858
CATATCC1055.460884E-419.999584
Produced by FastQC (version 0.11.5)