FastQCFastQC Report
Wed 25 May 2016
SRR1779620_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779620_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences476400
Sequences flagged as poor quality0
Sequence length76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT43830.9200251889168766No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT31800.6675062972292191No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG12560.26364399664147775No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12080.25356842989084805No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTATAC6200.059.256331
AGGGGGG8650.055.41682470
TCGTATG13400.049.88349540
CTCGTAT13000.049.52873239
CGTATGC12850.048.79650541
GTATGCC12750.048.36515442
TGAAAAA14950.047.06737559
CTTGAAA13900.046.8548457
TTGAAAA14550.046.67709758
TCTCGTA14050.046.57448238
CCGTCTT11850.046.42555247
TAGATCT13500.045.87505734
GAAAAAA16400.045.67614460
GTCTTCT12950.045.42986749
AGATCTC14400.044.9517335
TATGCCG13800.044.43597443
GCTTGAA14650.044.2264156
TGCTTGA13900.044.09774855
TCTTCTG13450.044.00138550
TCTGCTT13600.043.5252253