FastQCFastQC Report
Wed 25 May 2016
SRR1779619_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779619_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences274481
Sequences flagged as poor quality0
Sequence length76
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG151555.521329345200578No Hit
CTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTG9790.3566731394887078No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTAT200.006946929352.4990431
TTAAGGT200.006946929352.4990434
ACTAATG200.006946929352.49904321
CCGTATC2350.044.6800455
CGTATCA2400.042.29089756
GCCGTAT2400.042.29089754
GTATCAT2450.041.4278257
TATCATT2450.039.9992758
CTATAGA350.001254211939.999271
ACATTTC350.001254211939.9992714
TAGATCT2550.038.43067639
AGATCTC2700.037.59190840
TCATTAA2700.037.59190860
ATCATTA2700.037.59190859
CATTAAA2650.036.98045761
ATAGATC400.002416452934.999363
GTAGATC2950.034.40615538
TCTCGGT3400.031.91118243
CGCCGTA3200.031.7181753
TCGGTGG3800.031.3152245
Produced by FastQC (version 0.11.5)