FastQCFastQC Report
Wed 25 May 2016
SRR1779619_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779619_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences274481
Sequences flagged as poor quality0
Sequence length76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT54741.9943092600216408No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT37491.3658504595946532No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17300.6302804201383703No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG14330.5220762092822454No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC4460.1624884782553255No Hit
ATCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC4020.14645822479515885No Hit
ATTAAACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT2880.10492529537563619No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG150.002225046569.984514
ATTATAC9500.062.260721
GTATGAC1150.060.91158742
AGGGGGG10750.060.87025570
CGTCAGT308.374149E-658.3204332
CGTATGA1300.056.5774941
CGTCTTA252.3775456E-456.04887848
ATCGTAT850.053.5273239
GGGTACT200.00692249652.54582247
TAAGACG200.00692749152.53624343
GCGCTTA200.0069374952.51708655
GCTTATA200.006947499252.497951
ACGTTTA200.006952508452.48838427
GAAGTTA407.4747004E-752.48838426
ACAGATA900.050.54437633
TCGTATG19450.050.21284540
TATGACG1400.050.03451543
TGAAAAA20200.049.8899559
CTTGAAA19000.049.55069457
TTGAAAA19350.049.36892358