FastQCFastQC Report
Wed 25 May 2016
SRR1779618_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779618_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences379613
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG145803.8407536095971424No Hit
CTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTG9370.24683032456738838No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATACCG150.002223753670.019
GCCGTAT1800.044.7222254
CGTATCA2000.040.2556
GTAGATC1950.037.69230738
TAGATCT2150.035.81395339
CGCCGTA2150.035.81395353
CCGTATC2200.035.055
TCTGTAA501.7083302E-435.017
CTCGCTA501.7083302E-435.013
AATCGAT501.7083302E-435.022
GGTCATA552.9897073E-431.818181
ATCGATA552.9897073E-431.8181823
TAATCGA552.9897073E-431.8181821
TGTAGAT2750.030.54545637
TCTAGCA703.4847515E-530.03
TCGGTGG2600.029.61538345
CTTATAT604.9780804E-429.16666649
TCGCTAA604.9780804E-429.16666614
CGGTGGT2800.028.7546
GTGTAGA3050.028.68852436
Produced by FastQC (version 0.11.5)