FastQCFastQC Report
Wed 25 May 2016
SRR1779618_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779618_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences379613
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT53211.4016906691815085No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT34030.896439268412831No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15850.417530484993928No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG13520.3561521865689531No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC3960.10431676470510758No Hit
ATCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC3890.10247278149062335No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG10150.058.94909770
ATTATAC9450.057.7616841
TCGTATG17250.051.93440240
CCGTCTT16050.051.52449447
CTCGTAT16800.051.23572539
GCCGTCT16500.051.18113346
CGTATGC16800.051.0678141
GTATGCC16600.050.8593942
TATGCCG16400.050.625243
CTTGAAA17400.050.48774757
TCTCGTA17000.050.4271338
CGTCTTC17200.050.3205448
ATGCCGT16750.049.99223744
CCATAGG352.088247E-549.9860763
TTGAAAA17800.049.9430758
ATCTCGT17100.049.92761237
GTCTTCT17550.049.7097749
TCTGCTT17350.049.07115653
GCTTGAA17900.048.8883956
TGAAAAA19050.048.3194859
Produced by FastQC (version 0.11.5)