FastQCFastQC Report
Wed 25 May 2016
SRR1779617_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779617_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences599203
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG219103.65652374904665No Hit
CTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTG14860.2479960881370754No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGT150.002224456869.999422
GCCGTAT3750.036.39969654
CGTATCA4200.033.33305456
CCGTATC4500.031.88862255
CTTACGC604.981722E-429.16642662
GTGTATA1004.4190165E-727.9997671
TAGATCT5400.027.22199639
CGCCGTA5150.027.1842453
GTAGATC5700.027.01731938
TCTCGGT5450.026.97225243
GTATCAT5350.026.82220557
TCATTAA5500.026.09069360
CGGTGGT5850.025.7262846
AGATCTC5600.025.62478640
TCGGTGG5800.025.34461645
ACTGGTG1251.0984695E-725.199798
ATCATTA5750.024.95631259
CATTAAA5850.024.52970961
GTATAAG1752.0008883E-1023.99981
TATCATT6100.023.52439358
Produced by FastQC (version 0.11.5)