FastQCFastQC Report
Wed 25 May 2016
SRR1779617_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779617_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences599203
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT71181.187911275477593No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT56200.9379125271402179No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26560.44325545766626673No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG21320.355805962253193No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC6580.10981253431641698No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG15050.055.10051370
CGTATGA1650.053.04611641
ATTATAC12550.051.304341
TCGTATG25100.048.80710640
CGTATGC24050.047.89369241
CTCGTAT24250.047.486839
GTATGCC23550.047.30288742
TATGCCG23700.046.5600743
TCTCGTA24700.046.4760738
CCGTCTT22250.046.3073847
GCCGTCT23000.045.7115946
TTGAAAA25450.045.38147758
CTTGAAA25000.045.35837657
AATCTCG25400.045.32167436
GTCTTCT23850.045.23530249
ATGCCGT24100.045.07180444
CGTCTTC24000.044.53333348
TCTTCTG24400.044.50276650
ATCTCGT25850.044.40476237
TATCGTA1500.044.3317538
Produced by FastQC (version 0.11.5)