FastQCFastQC Report
Wed 25 May 2016
SRR1779616_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779616_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences362007
Sequences flagged as poor quality0
Sequence length76
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG195535.401276770891171No Hit
CTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTG11420.31546351313648635No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT1850.045.40540754
CGTATCA2050.040.9756156
GATTCGA556.686503E-638.18181643
TATCATT2250.037.33333258
GTATCAT2250.037.33333257
ATCATTA2350.037.23404359
CATTAAA2450.035.71428361
AGTTTAT400.002417251535.00000442
TCATTAA2500.035.00000460
AAGTGGT400.002417251535.00000411
CCGTATC2350.034.2553255
TAGATCT2550.032.94117739
TGTAGAT3000.031.537
TCTGCGA450.00430460131.1111138
AGATCTC2700.031.1111140
TCTCGGT2950.030.84745843
CGCCGTA2650.030.37735753
CGGTGGT3250.030.15384746
GTAGATC2850.029.47368438
GCACTAC604.9776305E-429.1666666