FastQCFastQC Report
Wed 25 May 2016
SRR1779616_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779616_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences362007
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT68551.8936097920758437No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT53441.4762145483374631No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19710.5444646098003629No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG19250.5317576731941647No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC4030.11132381418038878No Hit
ATTAAACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT3750.10358915711574637No Hit
ATCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC3740.10331291936343773No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTATAC11300.064.1082151
AGGGGGG13950.057.9428170
ATCGTAT1050.056.6606339
GTATGCC21000.055.0473742
CGTATGC21800.054.94795241
TATGCCG21200.054.52805743
TCGTATG22350.054.33425540
CCGTCTT19800.054.14497847
TCTCGTA21950.054.04891238
GCCGTCT20250.053.9723746
CTTGAAA21650.053.85774257
CTCGTAT21550.053.75297539
TGAAAAA23200.053.7234459
TTGAAAA22200.053.304758
GCTTGAA22050.052.7219356
TCTGCTT20800.052.7063453
CCTTAGT200.00694354552.50891549
TTGATAA200.006954936352.48715629
TTCGTTA200.006954936352.48715612
GACTAAG200.006954936352.48715626