FastQCFastQC Report
Wed 25 May 2016
SRR1779615_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779615_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences521817
Sequences flagged as poor quality0
Sequence length76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG167423.2084044789648476No Hit
CTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTG8470.16231744078862897No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGACG404.5916007E-543.749583
CGTATCA2000.041.999656
GTATCAT2450.034.28867357
TCATTAA2600.033.6567560
TAGATCT2450.032.85682739
ATCATTA2600.032.3104859
CATTAAA2750.031.82092561
GCCGTAT2650.031.6978154
TTTAGAC450.004306528331.1108132
CTATAGA450.004306528331.11081311
TCTAAGC450.004306528331.1108133
GCTCTAA802.4653818E-630.6247061
CCGTATC2950.029.66073255
TTACACC1004.4172157E-727.9997334
GTGTAGA3450.026.3765636
TCGGTGG3350.026.11915245
AGATCTC3150.025.55531340
TGTAGAT3750.025.1997637
TATCATT3350.025.07678858
ATTAAAA3500.025.00215762
Produced by FastQC (version 0.11.5)