FastQCFastQC Report
Wed 25 May 2016
SRR1779615_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779615_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences521817
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT47260.9056814937037314No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT31810.6096006837646148No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14940.28630726864015543No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG12060.2311155060107279No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTATAC9750.058.1471
CGTCTTA2100.055.03043748
CGTATGA1350.054.45890841
AGGGGGG9100.054.2243870
TCGTATG15750.052.88266440
CTCGTAT16250.051.04014239
GTATGCC16300.050.69802542
CGTATGC15500.050.5940841
CTTGAAA15000.049.93702357
TGAAAAA20250.049.6038559
TTGAAAA15900.049.30709558
TCTCGTA17200.049.23838838
CCGTCTT12250.049.17005547
GAAAAAA21900.048.90303860
GCCGTCT12700.047.69897546
CGGATCT20900.047.38676534
TATGCCG17300.047.36269843
ATCTCGT18150.047.046837
ATCTAGT2550.046.66117537
GTCTTCT10650.046.35478249
Produced by FastQC (version 0.11.5)