FastQCFastQC Report
Wed 25 May 2016
SRR1779614_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779614_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences522695
Sequences flagged as poor quality0
Sequence length76
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG205843.9380518275476137No Hit
CTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTG20410.39047628157912356No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA4000.050.74458756
GCCGTAT3950.048.72898554
TAGATCT4250.046.94066639
ATCATTA4050.046.67062459
CTGTGTA305.873988E-446.6706249
GCATAAC305.8767595E-446.6661571
CATTAAA4000.046.3833761
TCATTAA4100.046.1014760
CCGTATC4100.046.09264855
TATCATT4100.045.24773858
GTATCAT4300.044.77123657
AGATCTC4750.041.26270740
ATAAGCG350.001254617540.0033934
TGTAGAT5100.039.80348637
GTAGATC5050.038.80774338
CGCCGTA5100.037.74107753
TAGTGTA5100.036.37563334
ATTAAAA5300.035.66681362
AGTGTAG5450.035.32071335
TCTCGGT5850.034.70047443
Produced by FastQC (version 0.11.5)