FastQCFastQC Report
Wed 25 May 2016
SRR1779614_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779614_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences522695
Sequences flagged as poor quality0
Sequence length76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT68211.3049675240819216No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT53541.0243067180669414No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33700.6447354575804245No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG20060.3837802159959441No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC6070.11612890882828418No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTATG252.8355516E-670.0135940
ATTATAC11650.061.5704771
AGGGGGG12500.060.18288870
CGTATGA1600.059.11357541
TCGTATG22750.054.4721140
CGTATGC21350.053.98106441
CTCGTAT22950.053.3821639
CTAACGA200.00694459152.51019340
GTATGCC21750.052.35410342
TCTCGTA24350.051.31921838
CTTGAAA22250.051.1286357
CCGTCTT19650.051.0001847
TGAAAAA26100.050.9628759
TCTTCTG20250.050.83927550
TTGAAAA23000.050.67889858
GTCTTCT20100.050.52182849
TCTGCTT19800.050.2261953
TTATCTC24950.050.20616535
TATGCCG22500.050.1466143
GAAAAAA28700.049.75147660
Produced by FastQC (version 0.11.5)