FastQCFastQC Report
Wed 25 May 2016
SRR1779613_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779613_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences383802
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG171004.4554223271374305No Hit
CTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTG10850.2826978494119363No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAATG150.002223887569.99908462
GCCGTAT1900.047.89411554
CGTATCA2050.042.68237356
CCGTATC2150.042.3250355
TCTCGGT2350.040.21224243
TCGGTGG2500.039.19949345
AGATCTC2600.036.3456840
TAGATCT2650.035.65991239
GTAGATC2750.034.3631938
CGGTGGT3200.033.90580746
TGGTCGC3100.033.87052549
GTATCAT2800.033.7495657
CGCCGTA2700.033.70326653
ATCATTA2750.031.81776859
ACGATTC450.00430519531.11070654
GCGGATA450.00430519531.11070619
ATCTCGG3050.030.98320242
CTCGGTG3250.030.15345444
GGTGGTC3650.029.7256447
GTGGTCG3700.028.37800848
Produced by FastQC (version 0.11.5)