FastQCFastQC Report
Wed 25 May 2016
SRR1779613_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779613_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences383802
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT58591.5265683868244564No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT45471.1847254573973038No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19110.49791298638360404No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG17020.4434578246074799No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC4730.1232406292828073No Hit
ATCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC3950.10291765024674181No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATACT150.002220260770.0278953
CGTCTTA950.062.6728848
ATTATAC12300.056.626441
ATCGTAT509.804353E-1055.99312239
AGGGGGG14050.055.5447870
TATGCCG20700.054.6352943
CGTATGC21450.054.51344741
GCCGTAT900.054.4803446
CCGTCTT19050.054.41905647
TCGTATG22200.054.2275740
CTTGAAA21150.053.6244157
GTATGCC21100.053.5995542
CGTCTTC19200.053.4466848
TGCCGTA1050.053.36849645
TCTCGTA22700.053.34146538
CTCGTAT22050.053.32678639
ATGCCGT21400.052.84815244
GACGTAT200.00693043552.5346246
ATCTCGT23250.052.0796237
TCTTCTG19650.052.0173450