FastQCFastQC Report
Wed 25 May 2016
SRR1779612_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779612_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences474516
Sequences flagged as poor quality0
Sequence length76
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG223454.709008758398031No Hit
CTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTG16480.34730125011590757No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCATT3150.044.44444358
TAGATCT3600.042.77777539
CGTATCA3300.042.42424456
TCATTAA3400.042.20588360
GTATCAT3300.041.3636457
GCCGTAT3500.040.054
CCGTATC3550.038.45070655
CATTAAA3650.038.35616361
ATCATTA3850.038.18181659
GTAGACT501.7089731E-435.04
ATTAAAA4300.033.37209362
GTAGATC4650.032.36559338
TAGTGTA4550.032.30769334
TCTCGGT4850.031.75257743
TGTAGAT4700.031.27659637
TATAGTG450.004305937331.1111095
TAGATAA450.004305937331.1111095
AGATCTC4950.030.4040440
TTAAAAA5150.029.90291463
GTGTAGA4950.029.69696836