FastQCFastQC Report
Wed 25 May 2016
SRR1779612_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779612_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences474516
Sequences flagged as poor quality0
Sequence length76
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT84971.7906667003852346No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT50951.0737256488716924No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26430.5569885946943833No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG24010.5059892606361008No Hit
ATTAAACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT5210.10979608696018679No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTATAC12350.062.0461851
AGGGGGG14200.057.6587870
TTTAGTC200.0069259352.54517750
GTATGCC24250.051.57555442
TATCGTA950.051.56892838
TCGTATG25750.051.5154240
CGTATGC24950.050.9549141
CCGTCTT23600.050.63157747
TGAAAAA27200.050.18465459
TTCTGCT23050.049.99960752
CTCGTAT25700.049.97083739
TTGAAAA25300.049.66485258
GTCTTCT24150.049.60787249
GAAAAAA29650.049.56878760
TCTCGTA26650.049.37127338
TATGCCG25450.049.2813643
TCTTCTG24200.049.21587450
GCCGTCT24550.049.10051346
TGCTTGA23900.049.0904455
GCTTGAA25150.048.99205856
Produced by FastQC (version 0.11.5)