FastQCFastQC Report
Wed 25 May 2016
SRR1779611_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779611_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences513525
Sequences flagged as poor quality0
Sequence length76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG199193.878876393554355No Hit
CTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTG12670.24672606007497203No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA1950.043.07692356
CAGTAGT350.001255120340.04
ATCATTA2300.033.4782659
GTATCAT2550.032.94117757
CAGTATA450.004306263831.1111131
TCATTAA2700.029.81481460
TACTACG952.81947E-729.4736862
GGTAAGC604.9804995E-429.16666629
CGGTAAG604.9804995E-429.16666628
ATACAGC853.953388E-628.823531
TTGTGCA500.00720671328.0000022
AGTCAAG500.00720671328.00000211
TGTCCTA500.00720671328.00000228
GTAAAGG755.582138E-528.03
GCCGTAT2900.027.75862154
TTGTGCG1154.8934453E-827.3913042
TTCGGTA657.9533085E-426.92307726
TAGATCT3150.026.66666839
CATTAAA2850.025.78947461
CCGTATC3200.025.1562555
Produced by FastQC (version 0.11.5)