FastQCFastQC Report
Wed 25 May 2016
SRR1779611_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779611_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences513525
Sequences flagged as poor quality0
Sequence length76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT60141.1711211722895671No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT50710.987488437758629TruSeq Adapter, Index 5 (95% over 23bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21960.4276325397984519No Hit
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG17840.3474027554646804TruSeq Adapter, Index 5 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTATAC10400.057.8762361
AGGGGGG11650.056.16665670
CGTCTTA1200.052.54869548
CGTATGC19750.050.01128441
CTTGAAA19400.049.80116357
TCGTATG20250.049.77057640
TCTCGTA21100.049.25347538
TTGAAAA20000.049.1774658
GTCTTAA506.156915E-849.0358949
CTCGTAT20300.048.78129639
GTATGCC20200.048.72852742
TGAAAAA22850.048.553559
CACGTAT651.8553692E-1048.44980639
CCGTCTT18300.048.43285847
GCCGTCT18800.047.1447546
TCTGCTT17550.047.10458853
TATGCCG20750.047.099343
ATCTCGT21850.047.08242437
GAAAAAA24750.046.65991660
ACTGTAG305.880539E-446.6599161
Produced by FastQC (version 0.11.5)