FastQCFastQC Report
Wed 25 May 2016
SRR1779610_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779610_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences406025
Sequences flagged as poor quality0
Sequence length76
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG250116.1599655193645715No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCTTA200.006949201752.4992647
TGAATAC200.006949201752.499263
TGGTATG352.0852423E-549.99929418
TGTTACA404.586576E-543.75477235
CGTGTTA404.586576E-543.75477233
CTTGGTA404.5899124E-543.74938216
GTCCTAT350.001254896639.9994351
GAAACTA350.001254896639.99943563
GTGTTAC459.185482E-538.8931334
GGTATGT459.1921494E-538.8883419
TTACACC459.1921494E-538.888344
CGTATCA1600.034.99950856
GTGCTTT501.7086802E-434.99950824
ATCATTA1400.034.99950859
CCGTATC1650.031.81773655
TACCCTC552.9903176E-431.8177345
TTTACAC552.9903176E-431.8177343
CTAAAGG552.9903176E-431.81773426
TAGACAG450.004305508531.1106745
TTAGAGC450.004305508531.1106744