FastQCFastQC Report
Wed 25 May 2016
SRR1779610_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779610_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences406025
Sequences flagged as poor quality0
Sequence length76
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT146853.616772366233606No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG19920.49061018410196416No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC10630.26180653900621886No Hit
CTAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT9200.22658703281817621No Hit
CTTAAACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT6090.14999076411551013No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5520.13595221969090573No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG15750.061.320670
TAACACG252.393097E-455.9814821
CCTTATA200.006957910552.4826351
TTCTTAA200.006957910552.48263516
GTTCCTA200.006957910552.48263511
GTATGCC27300.052.3548742
TCGTATG28750.051.98462340
CGTATGC27950.051.8893341
TGAAAAA29450.051.5745959
TTGAAAA28000.051.37698758
GAAAAAA30850.051.2760660
TCTGCTT26450.051.25601653
CTTGAAA27850.051.157357
CCGTCTT26400.051.10660647
TATGCCG27700.051.09296843
CTCGTAT28400.051.023139
CGTATGA1450.050.7353741
TGCTTGA27250.050.51013655
TCTCGTA28550.050.14204838
GTCTTCT27300.050.04509449
Produced by FastQC (version 0.11.5)