FastQCFastQC Report
Wed 25 May 2016
SRR1779609_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779609_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences374888
Sequences flagged as poor quality0
Sequence length76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG196805.249567870937454No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCCTA200.006948400852.51
GCCGTAT652.1002625E-532.30769354
GTATTAG604.977964E-429.1666661
GTAGACT500.007204270527.99999811
TAGGGGA500.007204270527.9999984
TGTACCG500.007204270527.9999985
CTCGGAT657.9492776E-426.92307912
TACTACT657.9492776E-426.9230792
TCATTAA700.001225012725.00000260
CATTAAG700.001225012725.0000021
CGTATCA851.306722E-424.70588356
ATCATTA851.306722E-424.70588359
GACTAGG750.001830737723.3333341
CCGTATC901.9255014E-423.33333255
ATGGACA800.002663827521.8754
GTTGTAG1003.9268145E-421.01
ATAGGTA850.003786070720.58823663
GACGTTC850.003786070720.58823631
ATTCATG850.003786070720.58823656
CGCCGTA1055.457127E-420.053
Produced by FastQC (version 0.11.5)