##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1779609_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 374888 Sequences flagged as poor quality 0 Sequence length 76 %GC 59 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.37998548899938 32.0 27.0 32.0 13.0 32.0 2 29.243696784106188 32.0 32.0 32.0 22.0 32.0 3 28.83002390047161 32.0 27.0 32.0 22.0 32.0 4 27.779032671091098 32.0 27.0 32.0 13.0 32.0 5 28.62981743880839 32.0 27.0 32.0 13.0 32.0 6 32.511939565950364 37.0 32.0 37.0 13.0 37.0 7 32.35166236315913 37.0 32.0 37.0 13.0 37.0 8 32.649020507458225 37.0 32.0 37.0 13.0 37.0 9 32.74274716715392 37.0 32.0 37.0 13.0 37.0 10-11 32.2730428821408 37.0 32.0 37.0 13.0 37.0 12-13 32.14201174750859 37.0 32.0 37.0 13.0 37.0 14-15 33.128643754934814 37.0 34.5 37.0 17.5 37.0 16-17 33.178628016901044 37.0 34.5 37.0 17.5 37.0 18-19 32.41258055739314 37.0 32.0 37.0 13.0 37.0 20-21 32.890284564990075 37.0 34.5 37.0 17.5 37.0 22-23 32.82090117581784 37.0 34.5 37.0 17.5 37.0 24-25 32.21078162011054 37.0 32.0 37.0 13.0 37.0 26-27 32.777796301828815 37.0 34.5 37.0 17.5 37.0 28-29 32.22061389001515 37.0 32.0 37.0 13.0 37.0 30-31 32.33926399351273 37.0 32.0 37.0 13.0 37.0 32-33 32.28171080429355 37.0 32.0 37.0 13.0 37.0 34-35 32.214189304538955 37.0 32.0 37.0 13.0 37.0 36-37 32.92489356821237 37.0 34.5 37.0 17.5 37.0 38-39 32.74289254390645 37.0 34.5 37.0 17.5 37.0 40-41 32.09226488978042 37.0 32.0 37.0 13.0 37.0 42-43 32.135608501739185 37.0 32.0 37.0 13.0 37.0 44-45 31.756609974178957 37.0 32.0 37.0 13.0 37.0 46-47 32.144363116450776 37.0 32.0 37.0 13.0 37.0 48-49 32.652482874885294 37.0 32.0 37.0 13.0 37.0 50-51 32.90485158233926 37.0 32.0 37.0 13.0 37.0 52-53 31.798474477710677 37.0 32.0 37.0 13.0 37.0 54-55 31.99145878235633 37.0 32.0 37.0 13.0 37.0 56-57 31.784518309468424 37.0 32.0 37.0 13.0 37.0 58-59 31.27962884914961 37.0 29.5 37.0 13.0 37.0 60-61 31.043022182625208 37.0 27.0 37.0 13.0 37.0 62-63 30.732450758626577 37.0 27.0 37.0 13.0 37.0 64-65 30.620203900898403 37.0 27.0 37.0 13.0 37.0 66-67 30.753698437933462 37.0 27.0 37.0 13.0 37.0 68-69 31.11420210836303 37.0 27.0 37.0 13.0 37.0 70-71 31.15701089392032 37.0 27.0 37.0 13.0 37.0 72-73 31.411116120014512 37.0 29.5 37.0 13.0 37.0 74-75 31.78362337551482 37.0 32.0 37.0 13.0 37.0 76 31.49078924905572 37.0 32.0 37.0 13.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 11101 1 0.4381002000496288 11101 2 0.513646653724102 11101 3 0.06409269080774749 11101 4 -0.056968777168236784 11101 5 0.4044436629880508 11101 6 -0.920756471423207 11101 7 0.558642618352323 11101 8 -0.4449051366994681 11101 9 -0.7736896007410081 11101 10-11 0.20625642412593237 11101 12-13 -0.2263818250268912 11101 14-15 -0.044583660445049134 11101 16-17 -0.17663070726469954 11101 18-19 0.05799243126659093 11101 20-21 0.6939023594344533 11101 22-23 0.4343975818964694 11101 24-25 -0.7759496099823195 11101 26-27 -0.4088331845377695 11101 28-29 0.094757920500669 11101 30-31 -0.23152539312860654 11101 32-33 -0.5528945566167707 11101 34-35 -0.6253108424057245 11101 36-37 -0.9297282973777214 11101 38-39 -0.16711320205965308 11101 40-41 -0.05426756755940687 11101 42-43 0.1926588085470371 11101 44-45 -0.5909473955979756 11101 46-47 -0.8280831047779031 11101 48-49 -0.05894927999522537 11101 50-51 0.0011261924740182394 11101 52-53 0.10786359952138724 11101 54-55 -0.17290075726588938 11101 56-57 -0.7625471707316684 11101 58-59 -0.31961802382321736 11101 60-61 -0.286623363202434 11101 62-63 -0.02107467327238055 11101 64-65 -0.6414310217405728 11101 66-67 -0.15782785721579629 11101 68-69 -0.4085148230140092 11101 70-71 -0.19184580876797241 11101 72-73 -0.7040430167247997 11101 74-75 -1.3498582398452221 11101 76 -1.377812464761682 11102 1 -0.24433822359569035 11102 2 -0.29393955317245 11102 3 0.3776148582954306 11102 4 0.14977999130466912 11102 5 -0.6149652040070244 11102 6 -0.1820372596005484 11102 7 0.1277559188449331 11102 8 0.6637155529557077 11102 9 -0.7040590588690847 11102 10-11 -0.768300226120374 11102 12-13 -0.636492662465308 11102 14-15 -0.6366403106913552 11102 16-17 0.14138653411461632 11102 18-19 -0.6845075687334088 11102 20-21 -0.5047675913044642 11102 22-23 0.053720241994987816 11102 24-25 -0.7445949301793675 11102 26-27 -0.3089563372471318 11102 28-29 -0.10220020757815007 11102 30-31 -0.1800475606162948 11102 32-33 -0.5086457881438662 11102 34-35 -0.9511852266421812 11102 36-37 -0.539802189003332 11102 38-39 0.2940690639502037 11102 40-41 -0.5736025429288674 11102 42-43 -0.6444101569702028 11102 44-45 -0.12821340545018955 11102 46-47 -0.8026520702951423 11102 48-49 -0.5399345016700963 11102 50-51 0.301520281143965 11102 52-53 -0.9682817206756589 11102 54-55 -0.2419524814038212 11102 56-57 -0.04901268797305036 11102 58-59 -0.14682245732075572 11102 60-61 -0.6232612942369187 11102 62-63 -0.3051387126812486 11102 64-65 -0.009374371494267564 11102 66-67 -0.8854633251960919 11102 68-69 -0.3287857589746004 11102 70-71 -0.5916241338911234 11102 72-73 -0.5578114896312059 11102 74-75 -0.571077451667886 11102 76 0.24947817563240804 11103 1 0.3542849648101054 11103 2 0.9710846014169228 11103 3 0.8341124428364921 11103 4 0.03900704444476588 11103 5 0.1717980810171298 11103 6 0.4448226437810945 11103 7 1.5501230686033907 11103 8 0.26057545633734946 11103 9 0.5214723420970984 11103 10-11 0.6984389043144077 11103 12-13 0.50081410081971 11103 14-15 0.9959079984873895 11103 16-17 0.8347802539213731 11103 18-19 0.5531615133921903 11103 20-21 0.2549908628018116 11103 22-23 0.2694086477920905 11103 24-25 0.643400238902764 11103 26-27 0.565560570965431 11103 28-29 -0.12011083559747249 11103 30-31 0.09265775339336813 11103 32-33 -0.2950829862114972 11103 34-35 -0.38585913968565677 11103 36-37 -0.023836005428456986 11103 38-39 0.5270159238535825 11103 40-41 0.1260351382305558 11103 42-43 -0.05588358692189388 11103 44-45 -0.12103949240671596 11103 46-47 -0.9147897514545633 11103 48-49 -0.3580021345203406 11103 50-51 -0.10002561257583409 11103 52-53 -0.33510539217324364 11103 54-55 -0.15399354420575406 11103 56-57 0.06462257772743385 11103 58-59 -0.0059891239874190205 11103 60-61 0.5133932951350637 11103 62-63 0.7546922051931482 11103 64-65 0.6441642758487305 11103 66-67 0.27632411441743443 11103 68-69 0.8196925018949628 11103 70-71 -0.07135843340803305 11103 72-73 -0.8779322625780672 11103 74-75 -0.7347610265471118 11103 76 1.087835663555115 11104 1 0.7938984913801548 11104 2 -0.16901201694056667 11104 3 -0.5958392479847632 11104 4 -0.03345672367117558 11104 5 -0.7170908078717595 11104 6 -0.5696701098420931 11104 7 -0.6769300714932278 11104 8 0.057676905612709106 11104 9 -0.39157113616376904 11104 10-11 -0.11557075752133983 11104 12-13 0.2713455017858948 11104 14-15 0.24228481008159264 11104 16-17 -0.05652409390471291 11104 18-19 -0.9057756846754401 11104 20-21 0.06416960676316563 11104 22-23 -0.35136429906781075 11104 24-25 -0.1247157031262276 11104 26-27 -0.13975343869640255 11104 28-29 0.1286814349339238 11104 30-31 0.454976593973079 11104 32-33 0.18076242441651402 11104 34-35 -0.09117314934749743 11104 36-37 0.05104322162468122 11104 38-39 0.06566326684874468 11104 40-41 -0.3159938472766939 11104 42-43 0.09870578505878314 11104 44-45 0.4127769327140598 11104 46-47 -0.629765596865191 11104 48-49 -0.18892000891647598 11104 50-51 0.19224491882512496 11104 52-53 -0.03317302502348696 11104 54-55 -0.5380515152202463 11104 56-57 0.19747281927332594 11104 58-59 0.020580924321755845 11104 60-61 -0.4382975261209765 11104 62-63 -0.6776749445653074 11104 64-65 -0.6650144681126235 11104 66-67 -0.17778216577580253 11104 68-69 0.648194917353905 11104 70-71 0.2812985714228837 11104 72-73 -0.13155545098386412 11104 74-75 -0.42799774152295456 11104 76 -1.0860773799231502 11105 1 -0.01472283898030824 11105 2 -0.24606625000502902 11105 3 -0.43919509645570187 11105 4 -0.23388516706636864 11105 5 0.07091714893415357 11105 6 -0.2622182550756591 11105 7 0.1109685885281948 11105 8 1.0693716615529922 11105 9 0.25999071488656966 11105 10-11 0.5862020363230656 11105 12-13 0.5796498714713465 11105 14-15 0.7109275626118077 11105 16-17 0.5807417377345203 11105 18-19 -0.21802566828092296 11105 20-21 0.01699711457788311 11105 22-23 0.49011164470991986 11105 24-25 -0.07516054103908942 11105 26-27 -0.2090694594190765 11105 28-29 -0.046193420247831796 11105 30-31 0.20615153440633094 11105 32-33 -0.11895121800812092 11105 34-35 0.06721975073338626 11105 36-37 0.10687201914145561 11105 38-39 -0.09208478220364924 11105 40-41 -0.14385141170714633 11105 42-43 0.32832739959088997 11105 44-45 -0.574118382825759 11105 46-47 0.8075854862659426 11105 48-49 -0.4020159496338991 11105 50-51 -0.5937738365030896 11105 52-53 0.16385628837411303 11105 54-55 0.08439367244233154 11105 56-57 -0.36771178299567353 11105 58-59 -0.316698022931611 11105 60-61 0.26525680531059237 11105 62-63 0.31999703392508394 11105 64-65 0.9650487054288099 11105 66-67 -0.005508574064869265 11105 68-69 -0.38668168657640933 11105 70-71 -0.26544766330288994 11105 72-73 -0.4844856977760017 11105 74-75 -0.13735573221087094 11105 76 -0.9784856252725689 11106 1 -0.40374350993930364 11106 2 -0.8161421963442557 11106 3 0.6031434926566632 11106 4 -0.183832343497091 11106 5 0.00820660216037794 11106 6 -0.18926192920407203 11106 7 -0.016605314635242507 11106 8 0.11988295383676473 11106 9 -0.3085304245078504 11106 10-11 0.44101695449636935 11106 12-13 0.6202143859928384 11106 14-15 -0.5256821609116216 11106 16-17 -0.16706060244926846 11106 18-19 0.2914726074780418 11106 20-21 -0.30886450760402084 11106 22-23 0.022872224373841732 11106 24-25 0.767365422243536 11106 26-27 0.2528278037220346 11106 28-29 0.11737027700334579 11106 30-31 -0.18687575444889148 11106 32-33 -0.17975812294562843 11106 34-35 -0.3504253147479055 11106 36-37 -0.10541892909144224 11106 38-39 0.41265937232024186 11106 40-41 0.2560230077319261 11106 42-43 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0.08511929226069981 11109 40-41 -0.2903833648466758 11109 42-43 -0.023291435509015912 11109 44-45 0.1423117087050585 11109 46-47 -0.10567718445039276 11109 48-49 0.3811613887408569 11109 50-51 -0.219210312463332 11109 52-53 0.39426394142479637 11109 54-55 -1.2498177782074755 11109 56-57 0.0862955312050353 11109 58-59 0.1880748029532171 11109 60-61 -0.6842772759720788 11109 62-63 -0.09488757112873714 11109 64-65 -0.7511437778869592 11109 66-67 -0.5620386676618452 11109 68-69 0.7100041953633003 11109 70-71 0.617576779350884 11109 72-73 0.6019830309167382 11109 74-75 0.44603442047823805 11109 76 -0.8912524179749006 11110 1 -0.2842939758080796 11110 2 -0.2564174292786454 11110 3 0.1449157432511825 11110 4 0.8279215842250238 11110 5 -0.25000945179463585 11110 6 0.7811485811074164 11110 7 0.5159860073405085 11110 8 -0.5137180753628812 11110 9 -0.567643129665548 11110 10-11 0.4842218977734305 11110 12-13 0.4624232667382273 11110 14-15 -0.021042965558613957 11110 16-17 -0.3991665632305228 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-0.3066758151547475 21610 64-65 0.6248747451135088 21610 66-67 -0.5310110283763052 21610 68-69 -0.27148893918661443 21610 70-71 -0.1712884448451888 21610 72-73 0.3334075916408814 21610 74-75 -0.24737603824032206 21610 76 0.3175347127348793 21611 1 -0.23267155692902364 21611 2 0.3897641505312528 21611 3 -0.06701477133420042 21611 4 -0.3031829716582912 21611 5 0.05392368488186605 21611 6 0.727592370029079 21611 7 0.5399781410671594 21611 8 0.11001184925200391 21611 9 0.9027927929827655 21611 10-11 0.27669977387962774 21611 12-13 0.6421184486458031 21611 14-15 0.475859689308642 21611 16-17 0.7333309785590529 21611 18-19 0.4968813201554738 21611 20-21 0.6033805568436819 21611 22-23 0.5832572790320256 21611 24-25 0.4133680327836018 21611 26-27 0.375302922012132 21611 28-29 -0.059885392763334266 21611 30-31 0.34402651345777713 21611 32-33 -0.3787383807364577 21611 34-35 0.08298144002448993 21611 36-37 0.9214941072929577 21611 38-39 0.34295795283908603 21611 40-41 1.0791752348489112 21611 42-43 0.18614539858535295 21611 44-45 -0.06395414619093032 21611 46-47 0.13207015192708127 21611 48-49 0.6048803131447116 21611 50-51 0.3692980589217427 21611 52-53 0.18977383487989385 21611 54-55 0.5398067778554392 21611 56-57 0.25182064536027937 21611 58-59 -0.18191504991334995 21611 60-61 0.5281275946519735 21611 62-63 -0.03532389786643364 21611 64-65 0.5381256285057319 21611 66-67 0.560462600729835 21611 68-69 0.7651031299142872 21611 70-71 -0.06958709685408593 21611 72-73 0.20459591777619934 21611 74-75 -0.04820708129751239 21611 76 0.3065152126694457 21612 1 0.29490638072085673 21612 2 0.23282303675560811 21612 3 -0.12515492587723287 21612 4 -0.7466948288392139 21612 5 0.10604199023758198 21612 6 0.12386202097498966 21612 7 -0.3354814912270072 21612 8 -0.9057089074759475 21612 9 0.5566963368143689 21612 10-11 0.07777891057027375 21612 12-13 -0.5121581300912084 21612 14-15 -0.14967987903667535 21612 16-17 -0.13737245869114645 21612 18-19 0.36194926579896247 21612 20-21 0.05667125761639369 21612 22-23 0.49334254415326484 21612 24-25 -0.050314354639795056 21612 26-27 0.5016551735442434 21612 28-29 0.4645084255153762 21612 30-31 0.24729056888010348 21612 32-33 0.18723550682015855 21612 34-35 0.3310629747966658 21612 36-37 0.5859891779031372 21612 38-39 0.03644316466962039 21612 40-41 -0.10579678753318333 21612 42-43 -3.4540876876576476E-4 21612 44-45 0.2177937887944097 21612 46-47 0.3709990088415509 21612 48-49 0.6242381602003988 21612 50-51 0.48504546100008383 21612 52-53 0.7129952577416105 21612 54-55 0.054648238020639894 21612 56-57 0.48143695231471995 21612 58-59 0.7703897728950722 21612 60-61 0.1708933820220757 21612 62-63 0.17432171897343096 21612 64-65 0.16870116807407953 21612 66-67 0.18453667480390834 21612 68-69 -0.19141165825517703 21612 70-71 0.4228722689865734 21612 72-73 -0.24237983495494575 21612 74-75 0.6109189512098148 21612 76 0.14214004613600295 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 4.0 11 20.0 12 67.0 13 174.0 14 273.0 15 404.0 16 679.0 17 936.0 18 1331.0 19 1925.0 20 3022.0 21 4392.0 22 6014.0 23 7687.0 24 9219.0 25 10413.0 26 11467.0 27 12751.0 28 14420.0 29 16003.0 30 19027.0 31 23045.0 32 28861.0 33 39731.0 34 58048.0 35 73825.0 36 31150.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.0699517454593 16.485767711337015 8.069898396050009 27.374382147153675 2 28.306054074817013 20.022513390666013 26.341200571904146 25.33023196261283 3 28.20023100294222 19.273807840762682 22.72204691013559 29.803914246159508 4 23.706013529374108 14.894048355775592 24.10800025607648 37.29193785877382 5 25.097362412240454 28.593339877510083 26.868824822346944 19.440472887902523 6 43.5831501675167 26.26384413478159 12.637907855146072 17.515097842555644 7 38.424747789994825 24.127868205267735 14.08054715300118 23.36683685173626 8 34.91042658073878 27.726947781737476 15.5913232752182 21.77130236230554 9 31.119961161733638 14.569684812530676 14.743069930219157 39.56728409551653 10-11 36.45061991848232 20.075329164977273 19.01194490087706 24.462106015663345 12-13 34.467627664795884 19.011011288704893 18.834825334499904 27.686535711999316 14-15 33.26020037984678 18.561543714389366 16.303135869913145 31.875120035850706 16-17 34.177807771921216 20.9703964917522 16.93892575916007 27.912869977166515 18-19 33.229791297667575 21.586180405881223 18.02471671539233 27.159311581058876 20-21 33.69966496660336 20.69484752779497 18.00511085977679 27.600376645824888 22-23 34.17834126459102 20.562274599347006 17.25955485371631 27.99982928234566 24-25 34.31918332942105 20.445306331491008 16.97186893152088 28.263641407567057 26-27 34.03123624578204 20.183656321271854 18.016058257865744 27.769049175080358 28-29 33.851755526657996 20.715547997732653 18.311760194725085 27.120936280884266 30-31 34.001800540162044 20.525224233936846 18.193458037411222 27.279517188489883 32-33 33.590610516488276 21.068320496148846 18.33149944983495 27.009569537527927 34-35 33.67974883098107 20.570731980570145 18.663090477930684 27.086428710518103 36-37 33.672724944749945 19.88295123990861 19.1292564549537 27.315067360387747 38-39 33.1637715797785 19.9050116301402 20.141615629201254 26.78960116088005 40-41 32.8579907305525 19.49491514120903 20.369977659964654 27.277116468273814 42-43 32.46356245065193 18.931921000405456 21.058689528605875 27.545827020336738 44-45 31.259377813344003 19.51398752959221 22.067953719449168 27.158680937614616 46-47 30.929145410289756 19.707912373712112 22.20172718482211 27.161215031176017 48-49 30.144736561319647 19.716288598194662 22.849624421160453 27.28935041932524 50-51 29.475470007042105 20.460244126245705 22.681040737500265 27.383245129211925 52-53 28.936775783700732 21.98429931072747 22.12460788288769 26.95431702268411 54-55 28.966784746377584 21.543500992296366 21.586047032713772 27.903667228612278 56-57 28.914502464736135 21.81798297091398 21.614322144213737 27.653192420136147 58-59 29.169378587738205 21.531230660890717 21.865463818527132 27.433926932843946 60-61 29.315688952433796 21.480282110923795 21.2440782313651 27.959950705277308 62-63 29.847607005863114 21.023591171716202 21.4459862464856 27.68281557593508 64-65 30.263705426687437 21.301428693369754 20.746996436268965 27.687869443673847 66-67 30.43802415654809 21.15831928469303 19.6561372996735 28.74751925908538 68-69 30.626480442158726 21.57964512067604 19.49875696207934 28.295117475085892 70-71 30.66702588506434 22.159951772262648 19.721090032222957 27.451932310450054 72-73 31.215672989460824 22.623083755905114 19.146169379039552 27.015073875594513 74-75 31.99337400336635 23.430793812535512 18.929437403804346 25.646394780293797 76 32.90333594746136 23.13369931125729 18.51336139519747 25.449603346083876 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 1.5 3 4.0 4 5.0 5 4.0 6 5.5 7 9.5 8 11.0 9 9.0 10 12.0 11 20.5 12 24.0 13 38.5 14 51.0 15 67.0 16 114.0 17 143.0 18 168.0 19 209.0 20 298.5 21 372.0 22 449.5 23 568.5 24 705.0 25 800.0 26 946.0 27 1258.5 28 1589.0 29 1753.0 30 1898.5 31 2241.0 32 2693.0 33 2948.0 34 3159.0 35 3595.5 36 4046.5 37 4272.0 38 4539.5 39 5126.5 40 5655.5 41 6314.5 42 6764.0 43 7217.0 44 8048.5 45 8896.5 46 9366.0 47 9536.5 48 9943.0 49 10741.5 50 11304.0 51 11860.0 52 12893.5 53 13755.0 54 14139.0 55 14299.5 56 14531.0 57 14752.0 58 14902.0 59 14806.0 60 14258.5 61 13778.5 62 13750.0 63 13316.5 64 12605.0 65 11881.5 66 11098.0 67 10760.0 68 10392.5 69 9556.5 70 8684.0 71 8280.0 72 7895.0 73 7118.0 74 6361.0 75 5996.0 76 5589.5 77 4851.5 78 3965.0 79 3410.0 80 3115.5 81 2486.0 82 1857.5 83 1564.0 84 1329.0 85 957.5 86 717.0 87 613.0 88 514.5 89 387.5 90 349.0 91 345.0 92 351.0 93 352.0 94 392.5 95 504.5 96 577.0 97 701.5 98 1214.0 99 11018.5 100 20435.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 2.667463349053584E-4 2 0.0 3 2.667463349053584E-4 4 0.0 5 0.0 6 0.0 7 5.334926698107168E-4 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 8.002390047160752E-4 28-29 1.333731674526792E-4 30-31 1.333731674526792E-4 32-33 1.333731674526792E-4 34-35 2.667463349053584E-4 36-37 4.001195023580376E-4 38-39 0.0 40-41 1.333731674526792E-4 42-43 0.0 44-45 1.333731674526792E-4 46-47 1.333731674526792E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 5.334926698107168E-4 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 2.667463349053584E-4 74-75 2.667463349053584E-4 76 5.334926698107168E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 374888.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.83194924374958 #Duplication Level Percentage of deduplicated Percentage of total 1 75.96499736755315 50.00923851002329 2 12.635118097782073 16.63588906603942 3 6.538233952724121 12.912740571584832 4 2.927975194758548 7.7101725763321 5 1.2227858933828113 4.02491894345751 6 0.4384243407778596 1.7317397369587453 7 0.16779219458647363 0.7732261066259859 8 0.05109506188912866 0.26909500167130884 9 0.020582335020534876 0.12194777109507313 >10 0.02972389486718719 0.28922200924311064 >50 0.0012268749970660302 0.04522984061877786 >100 0.0016358333288163823 0.17822710999526145 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 4.0895833220409557E-4 5.298352756354601 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 19680 5.249567870937454 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 8.002390047160752E-4 0.0 2 0.0 0.0 0.0 0.0016004780094321504 0.0 3 0.0 0.0 0.0 0.0016004780094321504 0.0 4 0.0 0.0 0.0 0.004534687693391093 0.0 5 0.0 0.0 0.0 0.007202151042444677 0.0 6 0.0 0.0 0.0 0.007735643712255394 0.0 7 0.0 0.0 0.0 0.008002390047160753 0.0 8 0.0 0.0 0.0 0.008002390047160753 0.0 9 0.0 0.0 0.0010669853396214335 0.010403107061308977 0.0 10 0.0 0.0 0.0010669853396214335 0.013604063080173279 0.0 11 0.0 0.0 0.0010669853396214335 0.01493779475470007 0.0 12 0.0 0.0 0.0010669853396214335 0.015738033759416147 0.0 13 0.0 0.0 0.0010669853396214335 0.015738033759416147 0.0 14 0.0 0.0 0.0010669853396214335 0.019205736113185806 0.0 15 0.0 0.0 0.0010669853396214335 0.020806214122617954 0.0 16 0.0 0.0 0.0010669853396214335 0.022139945797144747 0.0 17 0.0 0.0 0.0010669853396214335 0.023206931136766182 0.0 18 0.0 0.0 0.0010669853396214335 0.02667463349053584 0.0 19 0.0 0.0 0.0010669853396214335 0.029075350504684065 2.667463349053584E-4 20 0.0 0.0 0.0010669853396214335 0.0301423358443055 2.667463349053584E-4 21 0.0 0.0 0.0010669853396214335 0.033076545528264445 2.667463349053584E-4 22 0.0 0.0 0.0010669853396214335 0.03894496489618233 2.667463349053584E-4 23 0.0 0.0 0.0010669853396214335 0.04081218924051983 2.667463349053584E-4 24 0.0 0.0 0.0010669853396214335 0.04908132562258594 2.667463349053584E-4 25 0.0 0.0 0.0010669853396214335 0.05121529630182881 2.667463349053584E-4 26 0.0 0.0 0.0010669853396214335 0.059484432683894924 2.667463349053584E-4 27 0.0 0.0 0.0010669853396214335 0.1251040310706131 2.667463349053584E-4 28 0.0 0.0 0.0010669853396214335 0.18965664411770983 2.667463349053584E-4 29 0.0 0.0 0.0010669853396214335 0.2643456178912102 2.667463349053584E-4 30 0.0 0.0 0.0010669853396214335 0.33476665030622477 2.667463349053584E-4 31 0.0 0.0 0.0010669853396214335 0.4235931798297091 2.667463349053584E-4 32 0.0 0.0 0.0010669853396214335 0.5310919527965686 2.667463349053584E-4 33 0.0 0.0 0.0010669853396214335 0.6874053050511086 2.667463349053584E-4 34 0.0 0.0 0.0010669853396214335 0.9128059580461364 2.667463349053584E-4 35 0.0 8.002390047160752E-4 0.0010669853396214335 1.2587759544183863 2.667463349053584E-4 36 0.0 8.002390047160752E-4 0.0010669853396214335 1.836815262158298 2.667463349053584E-4 37 0.0 8.002390047160752E-4 0.0010669853396214335 2.654926271313032 2.667463349053584E-4 38 0.0 8.002390047160752E-4 0.0010669853396214335 3.542658073878065 2.667463349053584E-4 39 0.0 8.002390047160752E-4 0.0010669853396214335 4.433857578796867 2.667463349053584E-4 40 0.0 8.002390047160752E-4 0.0010669853396214335 5.482704167644736 2.667463349053584E-4 41 0.0 8.002390047160752E-4 0.0010669853396214335 6.548089029256738 2.667463349053584E-4 42 0.0 8.002390047160752E-4 0.0010669853396214335 7.736710697595015 2.667463349053584E-4 43 0.0 8.002390047160752E-4 0.0010669853396214335 8.878385010989948 2.667463349053584E-4 44 0.0 8.002390047160752E-4 0.0010669853396214335 10.05873754294616 0.002667463349053584 45 0.0 8.002390047160752E-4 0.0010669853396214335 11.187074539595827 0.002667463349053584 46 0.0 8.002390047160752E-4 0.0010669853396214335 12.350622052453 0.002667463349053584 47 0.0 8.002390047160752E-4 0.0010669853396214335 13.368259320116941 0.002667463349053584 48 0.0 8.002390047160752E-4 0.0010669853396214335 14.245588015620665 0.002667463349053584 49 0.0 8.002390047160752E-4 0.0010669853396214335 15.103177482341392 0.003467702353769659 50 0.0 8.002390047160752E-4 0.0010669853396214335 15.925823179189518 0.0037344486886750176 51 0.0 8.002390047160752E-4 0.0010669853396214335 16.691385160367897 0.0037344486886750176 52 0.0 8.002390047160752E-4 0.0010669853396214335 17.492157657753783 0.0037344486886750176 53 0.0 8.002390047160752E-4 0.0010669853396214335 18.326006700667932 0.0037344486886750176 54 0.0 8.002390047160752E-4 0.0010669853396214335 19.1358485734406 0.0037344486886750176 55 0.0 8.002390047160752E-4 0.0010669853396214335 19.96516292866136 0.0037344486886750176 56 0.0 8.002390047160752E-4 0.0010669853396214335 20.761934231023666 0.0037344486886750176 57 0.0 8.002390047160752E-4 0.0010669853396214335 21.52882994387657 0.0037344486886750176 58 0.0 8.002390047160752E-4 0.0010669853396214335 22.252246004139902 0.0037344486886750176 59 0.0 8.002390047160752E-4 0.0010669853396214335 22.85695994537035 0.0037344486886750176 60 0.0 8.002390047160752E-4 0.0010669853396214335 23.420328204690467 0.0037344486886750176 61 0.0 8.002390047160752E-4 0.0010669853396214335 24.035978745652034 0.0037344486886750176 62 2.667463349053584E-4 8.002390047160752E-4 0.0010669853396214335 24.628422355476836 0.0037344486886750176 63 2.667463349053584E-4 8.002390047160752E-4 0.0010669853396214335 25.315827660527944 0.0037344486886750176 64 2.667463349053584E-4 8.002390047160752E-4 0.0010669853396214335 25.942148054885728 0.0037344486886750176 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTCCTA 20 0.0069484008 52.5 1 GCCGTAT 65 2.1002625E-5 32.307693 54 GTATTAG 60 4.977964E-4 29.166666 1 GTAGACT 50 0.0072042705 27.999998 11 TAGGGGA 50 0.0072042705 27.999998 4 TGTACCG 50 0.0072042705 27.999998 5 CTCGGAT 65 7.9492776E-4 26.923079 12 TACTACT 65 7.9492776E-4 26.923079 2 TCATTAA 70 0.0012250127 25.000002 60 CATTAAG 70 0.0012250127 25.000002 1 CGTATCA 85 1.306722E-4 24.705883 56 ATCATTA 85 1.306722E-4 24.705883 59 GACTAGG 75 0.0018307377 23.333334 1 CCGTATC 90 1.9255014E-4 23.333332 55 ATGGACA 80 0.0026638275 21.875 4 GTTGTAG 100 3.9268145E-4 21.0 1 ATAGGTA 85 0.0037860707 20.588236 63 GACGTTC 85 0.0037860707 20.588236 31 ATTCATG 85 0.0037860707 20.588236 56 CGCCGTA 105 5.457127E-4 20.0 53 >>END_MODULE