FastQCFastQC Report
Wed 25 May 2016
SRR1779609_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779609_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences374888
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT106912.851785066473187No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG15890.4238599261646145No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC9530.2542092571648065No Hit
CTAATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT7060.18832291244318303No Hit
CTTAAACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT5380.14350952817908283No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4380.11683489468854698No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCGA150.002226288269.9797210
TAACTCG601.8189894E-1258.31643736
CGTAACT700.054.98406634
AGGGGGG12300.054.61832470
AACGTAA200.006956364552.48479532
CGTATAT200.006956364552.48479534
TCGTAAG850.049.40404540
TTGAAAA21000.048.83221458
TCGTATG20550.048.70285440
CGTATGC19500.048.4927841
CCGTCTT18550.048.17638447
CTTGAAA21150.048.15492657
CTCGTAT20650.048.1280739
ACGTAAC800.048.1110633
GTATGCC19600.047.90078742
TATGCCG19650.047.4223443
GTCTTCT19900.047.354749
TCTCGTA21300.047.3165538
GCCGTCT19050.046.90564746
GCTTGAA21650.046.887456
Produced by FastQC (version 0.11.5)