FastQCFastQC Report
Wed 25 May 2016
SRR1779608_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779608_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences358474
Sequences flagged as poor quality0
Sequence length76
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG180125.024632190898084No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTTT200.006948155452.570
TGTATAA305.8739603E-446.6666642
TATCATT806.230766E-835.064
TTAGAGG400.002417220735.03
GTGTATA400.002417220735.01
TGCCTAG400.002417220735.010
CTAATGA400.002417220735.01
TTATGGT450.00430454631.11110923
GGTCATA450.00430454631.1111091
AGTGCGA450.00430454631.1111097
GGTCTTT450.00430454631.1111091
GTATCAT1052.0183506E-829.99999863
CTGTAGC604.977533E-429.1666661
TACAACA853.9490096E-628.823534
CCCCTAT500.007203855627.9999981
ATTAGAG500.007203855627.9999982
TAGCACC500.007203855627.9999984
CCATATG500.007203855627.9999988
GTATAAG500.007203855627.9999983
ATCATTA1004.41034E-727.99999865
Produced by FastQC (version 0.11.5)