FastQCFastQC Report
Wed 25 May 2016
SRR1779608_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779608_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences358474
Sequences flagged as poor quality0
Sequence length76
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT98122.7371580644621365No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG14120.39389188616189735No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC8800.24548502820288223No Hit
CTAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT6490.18104520829962562No Hit
CTTAAACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT4220.11772122943365489No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG11700.060.4066470
TCGTAAG650.059.21872740
GATCCTA200.00690781352.57734348
CCTTATA200.006953743352.4893261
GTCTGTA200.00695758152.4820027
ATTAGAG200.00695758152.4820023
TCGTATG22550.051.82981540
TCTCGTA22350.051.66734338
CTCGTAT22150.051.65992439
CTTGAAA20900.051.5828657
GTATGCC21550.051.5607742
TGAAAAA22100.051.15772259
TTGAAAA21400.051.03190258
CGTATGC22400.050.82765641
GAAAAAA23550.050.66910660
CCGTCTT21600.050.6300447
GTCTTCT21850.050.48967449
TTCTGCT21650.050.14726352
TCTTCTG22100.049.9185250
TCTGCTT21050.049.76734553
Produced by FastQC (version 0.11.5)