FastQCFastQC Report
Wed 25 May 2016
SRR1779607_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779607_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences497358
Sequences flagged as poor quality0
Sequence length76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG185113.721866341749806No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTA200.006950006452.4994747
AGTGCTA400.002418237534.9996510
TTACTCC500.00720685227.9997184
TATAGAA1202.1650176E-623.33312
TACACAG750.001831682923.33315
GACTGAT952.778523E-422.1050427
TAGAGAG10400.021.53824470
GTGTTAG850.003788006720.5880281
GGGACCG1408.23652E-619.9997985
ACCATAC900.00527304619.444253
TATATGA900.00527304619.444252
TACACCA1451.1151818E-519.310155
CTGTGCG1852.2657514E-718.918739
ATAGAGA15400.018.63617570
GACAGAG1702.1150645E-618.5292247
TTAGCCT1000.0096778317.50158363
TCTTGAT1000.00968343417.4998252
ACAGAGC1803.6503443E-617.4998258
ATGCACT1000.00968343417.49982511
GTCGTAA1250.001760815516.7998311
Produced by FastQC (version 0.11.5)