FastQCFastQC Report
Wed 25 May 2016
SRR1779607_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779607_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences497358
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT102752.0659163017383855No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG14750.29656706034687286No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC10040.20186666344966805No Hit
CTAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT7420.14918831103551164No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG13400.056.4067370
TCGTATG23200.052.037140
CGTATGC22700.051.6677241
CTCGTAT23100.051.20197339
GTATGCC23100.050.64185342
GTCTTCT22350.050.29388849
TCTTCTG22350.049.9805350
CCGTCTT22050.049.8915847
CTTGAAA23600.049.8306557
TTGAAAA24050.049.77145458
TATGCCG23450.049.7416543
TCTCGTA24100.049.65820738
TCTGCTT22950.048.97901553
GCCGTCT23200.048.92865446
TGCTTGA23400.048.9251555
TTCTGCT22650.048.70012352
GCTTGAA24100.048.65648356
TGAAAAA26350.048.34442559
ATCTCGT24850.047.87783437
ACAGATC25200.047.62944433
Produced by FastQC (version 0.11.5)