FastQCFastQC Report
Wed 25 May 2016
SRR1779606_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779606_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences251317
Sequences flagged as poor quality0
Sequence length76
%GC67

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG186707.428864740546799No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTATT150.002223227269.99721545
GATAGTT150.002223227269.99721518
GTCTATG252.3879005E-455.9977722
TCATTAA452.6888301E-854.45311460
TTACTAT200.006946841752.49791344
TCATACG200.006946841752.49791311
TGTGTAT200.006946841752.4979139
GCCAATT200.006946841752.49791320
GGTCTAT305.871741E-446.664811
ATCATTA602.5857662E-740.83983659
GTATAAG654.851445E-737.6908071
CATTAAA708.6532054E-735.0055761
CGTATCA708.6667023E-734.99860856
GCTCAGA400.002416321334.9986086
TATAAGA851.0597432E-732.9398652
TTAAAAA751.4838515E-632.67186763
AAGAGAG552.987547E-431.81691770
AGACTCT450.00430295831.10987344
AACTTAT450.00430295831.10987345
ACAGAGC450.00430295831.1098733
Produced by FastQC (version 0.11.5)