FastQCFastQC Report
Wed 25 May 2016
SRR1779606_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779606_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences251317
Sequences flagged as poor quality0
Sequence length76
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT106344.2313094617554725No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG15130.6020285137893577No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC11540.45918103431124835No Hit
CTAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT7080.28171592053064454No Hit
CTTAAACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT4830.19218755595522785No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3300.1313082680439445No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACAT150.002223854469.9922313
CTATACA150.002223854469.992231
GTTGTAC150.002223854469.992231
AGGGGGG13000.062.18541370
ATAACTC553.6379788E-1157.26638429
TCGTATG21900.053.54353340
TCTGCTT21500.053.24784553
GTATGCC21200.053.1968242
TATGCCG21150.053.15698643
TGCTTGA21750.053.0975655
CCGTCTT20400.053.0404947
TGAAAAA22650.052.9963359
CGTATGC21800.052.8362741
TTGAAAA22150.052.7706758
CTCGTAT22050.052.7030639
TTCTGCT21550.052.63692552
ATGGGGT200.00692694652.53598842
GTGTGTG200.006932403452.5255347
ATAGGTC200.006948795652.494183
ATTACCT200.006948795652.494185