FastQCFastQC Report
Wed 25 May 2016
SRR1779605_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779605_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences209871
Sequences flagged as poor quality0
Sequence length76
%GC68

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2746713.087563312701612No Hit
CTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTG3340.15914537978091303No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2710.12912693988211807No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2620.12483859132514734No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAATAG150.002222363870.037
GTAATAC150.002222363870.01
TAGCACT150.002222363870.029
GGTCTAT150.002222363870.01
ATCATTA850.057.6470659
TATCATT800.056.87558
CGGGTAT200.00694416152.542
CTAGAAC200.00694416152.53
CATAATA200.00694416152.536
TCATTAA1000.048.99999660
GCCGTAT1000.048.99999654
ATTAAAA1100.047.72727262
CATTAAA1050.046.66666861
TAAACCA305.868363E-446.6666642
CGTATCA1000.045.49999656
GTATCAT1050.043.33333657
TAGATCT1150.042.608739
GTATAAG503.4672576E-642.01
TCACACC459.176213E-538.8888854
TATAAGA556.673081E-638.1818162
Produced by FastQC (version 0.11.5)